Candida guilliermondii
On Sabouraud's dextrose agar colonies are white to cream colored, smooth, glabrous and yeast-like in appearance. Microscopic morphology shows spherical to subspherical budding yeast-like cells or blastoconidia, 2.0-4.0 x 3.0-6.5 um in size.
India Ink Preparation: Negative - no capsules present.
Dalmau Plate Culture on Cornmeal and Tween 80 Agar: Branched pseudohyphae with dense verticils of blastoconidia.
Physiological Tests:
Germ Tube test is Negative
Hydrolysis of Urea is Negative
Growth on Cycloheximide medium is Positive
Growth at 37C is Positive
Fermentation Reactions: Where fermentation means the production of gas and is independent of pH changes.
Positive: Glucose; Sucrose;
Variable: Galactose.
Negative: Lactose; Maltose; Trehalose.
Assimilation Tests:
Positive: Glucose; Sucrose; Galactose; Maltose; Trehalose; D-Xylose; Melezitose; Glycerol; Raffinose; Cellobiose; Melibiose; L-Rhamnose; D-Arabinose (delayed); L-Arabinose; Ribitol; L-Sorbose (delayed); Galactitol (delayed); D-Mannitol; D-Glucitol; Salicin; Citric acid; Succinic acid.
Variable: D-Ribose; DL-Lactic acid.
Negative: Potassium nitrate; Lactose; Erythritol ; Inositol; Soluble Starch.
MIC data is limited. Antifungal susceptibility testing of individual strains is recommended.
| Antifungal | MIC ug/mL | Antifungal | MIC ug/mL |
||
Range |
MIC90 |
Range |
MIC90 |
||
| Fluconazole | 0.125->128 |
16 |
Amphotericin B | 0.03-1 |
0.5 |
| Itraconazole | 0.03-8 |
1 |
Flucytosine | 0.03-8 |
0.125 |
| Posaconazole | 0.03-8 |
0.05 |
Caspofungin | 0.125->8 |
0.5 |
| Voriconazole | 0.03-8 |
0.25 |
Anidulafungin | 0.06-4 |
nd |
Clinical significance:
Candida guilliermondii has been isolated from numerous human infections, mostly of cutaneous origin. Systemic infections are rare, although one has been reported in a patient with aplastic anemia. C. guilliermondii has also been isolated from normal skin and in sea water, faeces of animals, fig wasps, buttermilk, leather, fish and beer.
Mycosis: Candidiasis
Further reading:
Kreger-Van Rij, N.J.W. (ed) 1984. The Yeasts: a taxonomic study. 3rd Edition. Elsevier Science Publishers B.V., Amsterdam, The Netherlands.
Rippon, J.W. 1988. Medical Mycology. 3rd Edition. W.B. Saunders Co., Philadelphia, USA.
