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School of Molecular & Biomedical Science
The University of Adelaide
AUSTRALIA 5005

Contact:
Dr David Ellis
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Telephone:
 +61 8 8161 6459
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 +61 8 8161 7589

Dimorphic Fungi

Disclaimer: Please note that some of the images within this gallery are somewhat explicit and are not recommended for viewing if you are squeamish or under the age of 18.

 

  • "Blastomycosis" - Ulcerated granuloma due to B. dermatitidis. (Courtesy of Dr. John Rippon, USA).
  • "Blastomycosis" - Cutaneous blastomycosis of 20 years duration showing loss of skin. Note advancing edge of lesion. (Courtesy of Dr. John Rippon, USA).
  • "Blastomycosis" - Cutaneous blastomycosis showing loss of skin from the arm and fingers. Note advancing edge of lesion. (Courtesy of Dr. John Rippon, USA).
  • "Blastomycosis" - Cutaneous blastomycosis showing loss of skin from the arm and fingers. Note advancing edge of lesion. (Courtesy of Dr. John Rippon, USA).
  • "Blastomycosis" - Tissue sections showing large, broad-base, unipolar budding yeast-like cells of Blastomyces dermatitidis, 8-15mm in diameter. Note: tissue sections need to be stained by Grocott's methenamine silver method to clearly see the yeast-like cells, which are often difficult to observe in Haematoxylin and eosin (H&E) stained preparations.
  • "Blastomycosis" - Tissue sections showing large, broad-base, unipolar budding yeast-like cells of Blastomyces dermatitidis, 8-15mm in diameter. Note: tissue sections need to be stained by Grocott's methenamine silver method to clearly see the yeast-like cells, which are often difficult to observe in Haematoxylin and eosin (H&E) stained preparations.
  • "Blastomycosis" - H& E stain of lung tissue containing abundant broad-based budding yeast cells morphologically consistent with Blastomyces dermatitidis. (1000X magnification). Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Blastomycosis" - Typical colony morphology of Blastomyces dermatitidis. Thallus develops slowly, developing a fine white to tan aerial mycelium. Reverse is tan to brown in color. Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Blastomycosis" - Typical colony morphology of Blastomyces dermatitidis. Reverse is tan to brown in color. Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Blastomycosis" - Calcofluor white stain of mould phase growth at 30o C. Septate hyphae bearing round or pear-shaped terminal conidia attached to conidiophores of varying lengths or directly on the hyphae are observed. (600X magnification). Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Blastomycosis" - Calcofluor white stain of mould phase growth at 30o C. Septate hyphae bearing round or pear-shaped terminal conidia attached to conidiophores of varying lengths or directly on the hyphae are observed. (600X magnification). Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Blastomycosis" - Calcofluor white stain of yeast phase growth at 36o C. Large, globose, yeastlike cells are apparent, some with broad bases. (1000X magnification). Image courtesy of Jon Biehle, Dept. of Pathology, Creighton University, Omaha, NE, USA.
  • "Coccidioidomycosis" - Coccidioidomycosis showing chronic lesions of the face. Active lesions are seen on the cheek. An atrophic, depigmented scar representing a healed lesion is on the forehead. (Courtesy of Dr J.W. Rippon, USA).
  • "Coccidioidomycosis" - Chronic cutaneous coccidioidomycosis showing granulomatous lesions on face, neck and chin. (Courtesy of Dr J.W. Rippon, USA).
  • "Coccidioidomycosis" - Extension of pulmonary coccidioidomycosis showing a large superficial, ulcerated plaque. (Courtesy of Dr J.W. Rippon, USA).
  • "Coccidioides immitis." - Direct microscopy of skin scrapings from a cutaneous lesion mounted in 10% KOH and Parker ink solution showing characteristic endosporulating spherules (sporangia) of Coccidioides immitis. The presence of spherules with endospores is diagnostic.
  • "Coccidioides immitis" - Periodic Acid-Schiff (PAS) stained tissue section of viscera from an experimentally induced infection in a mouse showing typical endosporulating spherules of Coccidioides immitis. Young spherules have a clear centre with peripheral cytoplasm and a prominent thick wall. Endospores (sporangiospores) are later formed within the spherule by repeated cytoplasmic cleavage. Rupture of the spherule releases endospores into the surrounding tissue where they re-initiate the cycle of spherule development.
  • "Coccidioides immitis" - Culture of Coccidioides immitis on Sabouraud's dextrose agar showing a suede-like to downy, greyish white colony with a tan to brown reverse.
  • "Coccidioides immitis" - Microscopic morphology of Coccidioides immitis showing typical single-celled, hyaline, rectangular to barrel-shaped, alternate arthroconidia, separated from each other by a disjunction cell. This arthroconidial state has been classified in the genus Malbranchea and is similar to that produced by many non-pathogenic soil fungi, e.g. Gymnoascus sp.
  • "Coccidioides immitis" - Microscopic morphology of Coccidioides immitis showing typical single-celled, hyaline, rectangular to barrel-shaped, alternate arthroconidia, separated from each other by a disjunction cell. This arthroconidial state has been classified in the genus Malbranchea and is similar to that produced by many non-pathogenic soil fungi, e.g. Gymnoascus sp.
  • "Histoplasmosis" - Histoplasmosis of the lower gum showing ulcer around base of tooth.
  • "Histoplasmosis" - Tissue section stained with haematoxylin and eosin from a biopsy of the mouth lesion shown in the previous slide. Note macrophages containing numerous yeast cells of Histoplasma capsulatum. The basophilic cytoplasm of the fungal cells is retracted from the poorly stained cell wall, giving the false impression of a capsule. (Courtesy Dr G. Hunter, Adelaide, S.A.).
  • "Histoplasma capsulatum" - Culture of Histoplasma capsulatum on Sabouraud's dextrose agar showing a white suede-like colony with a pale yellow-brown reverse.
  • "Histoplasma capsulatum" - Culture of Histoplasma capsulatum on Sabouraud's dextrose agar showing a white suede-like colony with a pale yellow-brown reverse.
  • "Histoplasma capsulatum" - Culture of Histoplasma capsulatum on Sabouraud's dextrose agar showing a white suede-like colony with a pale yellow-brown reverse.
  • "Histoplasma capsulatum" - Microscopic morphology of the saprophytic or mycelial form of Histoplasma capsulatum showing characteristic large, rounded, single-celled, tuberculate macroconidia formed on short, hyaline, undifferentiated conidiophores.
  • "Histoplasma capsulatum" - Yeast-like culture of Histoplasma capsulatum on blood agar incubated at 37C.
  • "Histoplasma capsulatum" - Microscopic morphology of the parasitic or yeast form of Histoplasma capsulatum cultured on brain heart infusion agar containing blood incubated at 37C. Note the small round to oval budding yeast-like cells.
  • "Histoplasma capsulatum var. duboisii" - Tissue section stained by Periodic Acid-Schiff (PAS) showing numerous yeast cells of Histoplasma capsulatum var. duboisii. This African variant differs by having larger (7-15mm) budding yeast cells in vivo.
  • "Histoplasma capsulatum var. duboisii" - Tissue section stained by Grocott’s methenamine silver (GMS) showing numerous yeast cells of Histoplasma capsulatum var. duboisii. This African variant differs by having larger (7-15mm) budding yeast cells in vivo.
  • Exoantigen test for Histoplasma - Immy exoantigen immunodiffusion test kit for the identification of Histoplasma capsulatum, Coccidioides immitis and Blastomyces dermatitidis.
  • Exoantien test for Histoplasma - Exoantigen immunodiffusion plate showing positive identification of Histoplasma capsulatum. Note H and M bands of identification; EX = culture filtrate; H = Histoplasma antibody and antigen, C = Coccidioides antibody and antigen; B = Blastomyces antibody and antigen.
  • "Penicillium marneffei" infection. - Typical papules often with a central necrotic umbilication "Molluscum contagiosum" like lesions caused by P. marneffei in an HIV+ patient. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - "Molluscum contagiosum" like lesions caused by P. marneffei on the neck of an HIV+ patient. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - "Molluscum contagiosum" like lesions caused by P. marneffei in the buccal cavity of an HIV+ patient. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - "Molluscum contagiosum" like lesions caused by P. marneffei below the eye and on the cornea of an HIV+ patient. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - Typical papules "Molluscum contagiosum" like lesions caused by P. marneffei on the back of an HIV+ patient. (Courtesy Dr B. Curry, Darwin N.T.).
  • "Penicillium marneffei" infection. - Papule like lesions caused by P. marneffei that have been scratched in an HIV+ patient. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - Grocott’s methenamine silver (GMS) stained tissue section showing numerous small yeast-like cells of P. marneffei that closely resemble those seen in Histoplasmosis. (Courtesy Dr P. Jones, Sydney, N.S.W.).
  • "Penicillium marneffei" infection. - A Giemsa stained touch smear showing the typical septate yeast-like cells of P. marneffei. (Courtesy Dr B. Curry, Darwin N.T.).
  • "Penicillium marneffei" - Culture of P. marneffei showing distinctive red diffusible pigment. On Sabouraud's dextrose agar at 25OC, colonies are fast growing, suede-like to downy, white with yellowish-green conidial heads. Colonies become greyish-pink to brown with age and produce a diffusible brownish-red to wine red-pigment.
  • "Penicillium marneffei" - Microscopic morphology of P. marneffei showing hyaline, smooth-walled conidiophores bearing terminal verticils of 3 to 5 metulae, each bearing 3 to 7 phialides. Conidia are globose to subglobose, 2 to 3mm in diameter, smooth-walled and are produced in basipetal succession from the phialides.
  • "Penicillium marneffei" - Scanning electron micrograph showing phialides and conidia of P. marneffei.
  • "Sporotrichosis" - Lymphocutaneous sporotrichosis showing typical elevated subcutaneous nodules developing along the regional lymphatics of the forearm. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporotrichosis" - Lymphocutaneous sporotrichosis showing more advanced, ulcerating lesions developing along the lymph system of the forearm. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporotrichosis" - Lymphocutaneous sporotrichosis showing more advanced, ulcerating lesions developing along the lymph system of the forearm. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporotrichosis" - Fixed cutaneous sporotrichosis showing an ulcerating lesion on the leg. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporotrichosis" - Fixed cutaneous verrucous-type sporotrichosis of the wrist and hand, looking remarkably similar to chromoblastomycosis. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporotrichosis" - Section from a fixed cutaneous lesion on the face of a child with sporotrichosis showing round Periodic Acid-Schiff (PAS) positive yeast-like cells, one with an elongated bud. Sporothrix schenckii is a dimorphic fungus and this is the typical parasitic or yeast-like form seen in tissue. (Courtesy Professor D. Weedon, Brisbane, Qld.).
  • "Sporothrix schenckii" - Sporothrix schenckii on Sabouraud's dextrose agar grown at 25oC colonies are moist and glabrous, with a wrinkled and folded surface. Pigmentation may vary from white to cream to black.
  • "Sporothrix schenckii" - Microscopic morphology of the saprophytic or mycelial form of Sporothrix schenckii when grown on Sabouraud's dextrose agar at 25oC. Note clusters of ovoid conidia produced sympodially on short conidiophores arising at right angles from the thin septate hyphae.
  • "Sporothrix schenckii" - Microscopic morphology of the parasitic or yeast form of Sporothrix schenckii when grown on brain heart infusion agar containing blood and incubated at 370C. Note budding yeast cells.