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Bird Seed Agar

For the selective isolation of Cryptococcus neoformans and C. gattii.

Ingredients

  • Guizotia abyssinica (niger seed) 50 g
  • Glucose 1 g
  • KH2PO4 (potassium dihydrogen orthophosphate) 1 g
  • Creatinine 1 g
  • Bacto-agar (BD) 15 g
  • Distilled water 1000 mL
  • Additives: to each 500 ml bottle.
  • Penicillin G (20 units/ml) 1 mL
  • Gentamicin (40 mg/ml) 1 mL

Method

  1. Grind seeds of Guizotia abyssinica as finely as possible with an electric grinder and add to 1000 ml distilled water in a stainless steel jug.
  2. Boil for 30 minutes, pass through filter paper and adjust volume to 1000 mL.
  3. Add remaining ingredients except Bacto-Agar to filtrate and dissolve.
  4. If required: Cool to room temperature and pH to 5.5
  5. Dispense into 500 mL bottles.
  6. Add 7.5 g Bacto-agar to each 500 mL reagent bottle.
  7. Autoclave at 121C for 20 minutes.
  8. Cool to 48C and add 0.5 ml Penicillin G and 0.5 ml Gentamicin to each 500 ml of Bird Seed Agar.
  9. Mix gently and pour into 90 mm plastic petri dishes.
School of Biological Sciences
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THE UNIVERSITY OF ADELAIDE
SA 5005 AUSTRALIA

Contact

Dr David Ellis
Email