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Bromocresol Purple Milk Solids Glucose Agar (BCP)

For the differentiation of Trichophyton species
(Fischer and Kane 1971; Summerbell et al. 1988).

Solution A

  • Distilled water 1000 mL
  • Skim milk powder (Carnation Brand) 80 g
  • Bromcresol (or bromocresol) purple (1.6% solution in alcohol) 2 mL
  • Dissolve in 2 litre flask and autoclave 121C for 10 minutes.

Solution B

  • Glucose 40 g
  • Distilled water 200 mL
  • Dissolve and autoclave at 121C for 10 minutes.

Solution C

  • Bacto agar (BD 214010) 30 g
  • Distilled water 800 mL
  • Soak for 15 minutes in 3 litre flask; autoclave at 121C for 10 minutes.

Final mixture

  • To make media add solution A and B to solution C. Adjust final pH to 6.6.
  • Aseptically dispense for slopes (7 mL amounts into bottles).
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