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Creatinine Dextrose Bromothymol Blue
Thymine (CDBT) Agar

For differentiation of C. neoformans var. neoformans and C. neoformans var. grubii (Irokanulo et al. 1994).

Solution A

  • Creatinine 1 g
  • Dextrose 0.5 g
  • KH2PO4 1 g
  • MgSO4.7H2O 0.5 g
  • Thymine 0.1 g
  • Distilled water 980 mL
  1. Dissolve ingredients in small beaker and adjust pH to 5.6
  2. Store in refrigerator.

Solution B (Aqueous Bromothymol Blue)

  • Bromothymol Blue 0.4 g
  • 0.01N NaOH 64 mL
  • Distilled water 36 mL
  1. Dissolve the Bromothymol Blue in the NaOH
  2. Add to the water.

To prepare medium (1 litre for plates):

  • Solution A 980 mL Solution B 20 mL
  • Bacto agar (BD 214010) 20 g
  • Autoclave to 121C for 15 minutes, cool to 48C and dispense as plates.
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