Dixon's Agar for Malassezia furfur
For primary isolation and cultivation of Malassezia furfur.
- Malt extract (Oxoid L39) 9 g
- Bacto Tryptone 1.5 g
- Ox-bile Desiccated (Oxoid L50) 5 g
- Tween 40 2.5 ml
- Oleic acid 0.5 g
- Glycerol 0.5 mL
- Bacto agar (BD) 3 g
- Distilled water 250 mL
- Soak ingredients for 15 minutes in some water.
- Bring remaining water to boil, add to other ingredients. Bring to boil once more.
- Dispense for slopes.
- Autoclave at 121C for 10 minutes and then slope.