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Cellotape Flag Preparations

An excellent technique for the rapid mounting of sporulating fungi because it keeps more of the reproductive structures intact.

  1. Using clear 2cm wide cellotape and a wooden applicator stick (orange stick) make a small cellotape flag (2x2 cm).
  2. Using sterile technique, gently press the sticky side of the flag onto the surface of the culture.
  3. Remove and apply a drop of 95% alcohol to the flag, this acts as a wetting agent and also dissolves the adhesive glue holding the flag to the applicator stick.
  4. Place the flag onto a small drop of Lactophenol cotton blue on a clean glass slide, remove the applicator stick and discard, add another drop of stain, cover with a coverslip, gently press and mop up any excess stain.
Steps 1 to 4
School of Biological Sciences
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THE UNIVERSITY OF ADELAIDE
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Dr David Ellis
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