Rice Grain Slopes
To induce sporulation and for differentiation of M. audouinii and M. canis.
- Place approximately 1/2 teaspoon rice grains into wide neck 20ml vials.
- Add 8ml distilled water to each vial.
- Lid, then slope on racks ensuring rice grains are evenly distributed.
- Autoclave racks at 121C for 15 minutes.