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Sabouraud's Dextrose Agar (SDA) with Cyclohgeximide (0.05%), Chloramphenicol, Gentamicin and Yeast Extract.

For the primary isolation and cultivation of dermatophytes.

  • Sabouraud's Dextrose Agar (Oxoid CM41) 65 g
  • Cycloheximide (Actidione) 0.5 g
  • Chloramphenicol 0.05 g
  • Yeast extract 5 g
  • Gentamicin (40 mg/ml) 0.56 mL
  • Distilled water 1000 mL

Method

  1. Soak all ingredients, except Gentamicin, in 100 mL water.
  2. Boil remaining water, add to soaking ingredients, and bring to boil to dissolve, stirring well to prevent from charring.
  3. Add the Gentamicin. Mix well.
  4. Dispense for slopes if required.
  5. Autoclave at 121C for 10 minutes. Remove and slope, or pour for plates as required.
School of Biological Sciences
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THE UNIVERSITY OF ADELAIDE
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Dr David Ellis
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