Mould Identification: A Virtual Self Assessment
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Please find additional information below
Unknown 8 = Blastomyces dermatitidis
|A 42-year-old exchange teacher from Chicago USA presented with a three week history of pulmonary disease characterized by fever, dyspnea, cough, yellowish sputum, shortness of breath, chest pain and weight loss. CXR showed interstitial and diffuse bilateral infiltrates and a fine needle biopsy was taken which showed the presence of fungal elements and the culture shown below was isolated.|
|Direct Microscopy (H&E staining)|
H&E stain of lung tissue containing abundant broad-based budding yeast cells morphologically consistent with Blastomyces dermatitidis. (1000X magnification)
Typical colony morphology of Blastomyces dermatitidis. Thallus develops slowly, developing a fine white to tan aerial mycelium. Reverse is tan to brown in color.
Calcofluor white stain of mould phase growth at 30C. Septate hyphae bearing round or pear-shaped terminal conidia attached to conidiophores of varying lengths or directly on the hyphae are observed. (600X magnification).
Calcofluor white stain of yeast phase growth at 36C. Large, globose, yeastlike cells are apparent, some with broad bases. (1000X magnification).
What is your identification?