Mould Identification: A Virtual Self Assessment
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Unknown 29 = Aspergillus nidulans
|A 6 year old boy with chronic granulomatous disease presented with chronic pulmonary infiltrates. A needle biopsy showed the presence ofhyphal elements in a Grocott’s methenamine silver (GMS) stained tissue section and the culture shown below was grown.|
|Direct Microscopy (GMS)|
Grocott’s methenamine silver (GMS) stained tissue section of lung showing dichotomously branched, septate hyphae typical of Aspergillus.
Aspergillus nidulans on Czapek dox agar showing typical plain green colony with dark red-brown cleistothecia developing within and upon the conidial layer. Reverse may be olive to drab-grey or purple-brown.
Conidial heads of Aspergillus nidulans are short columnar and biseriate. Conidiophores are usually short, brownish and smooth-walled. Conidia are globose and rough-walled.
Cleistothecium of Emericella nidulans (anamorph A. nidulans) showing numerous reddish-brown ascospores .
Cleistothecia of A. nidulans are often surrounded by a mass of Hülle cells which are up to 25 um in diameter.
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