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Mould Identification: A Virtual Self Assessment

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Unknown 29 = Aspergillus nidulans

Case History
A 6 year old boy with chronic granulomatous disease presented with chronic pulmonary infiltrates.  A needle biopsy showed the presence ofhyphal elements in a Grocott’s methenamine silver (GMS) stained tissue section and the culture shown below was grown.
Direct Microscopy (GMS)

Grocott’s methenamine silver (GMS) stained tissue section of lung showing dichotomously branched, septate hyphae typical of Aspergillus.
Culture
culture

Aspergillus nidulans on Czapek dox agar showing typical plain green colony with dark red-brown cleistothecia developing within and upon the conidial layer. Reverse may be olive to drab-grey or purple-brown.
Microscopy

Conidial heads of Aspergillus nidulans are short columnar and biseriate. Conidiophores are usually short, brownish and smooth-walled. Conidia are globose and rough-walled.

Cleistothecium of Emericella nidulans (anamorph A. nidulans) showing numerous reddish-brown ascospores .

Cleistothecia of A. nidulans are often surrounded by a mass of Hülle cells which are up to 25 um in diameter.

Comment: Aspergillus nidulans is a typical soil fungus with a world-wide distribution. It has also been reported as a causative agent of aspergillosis in humans and animals.

About Aspergillus Back to Virtual Assessment

What is your identification?

Neosartorya fischeri
Aspergillus flavipes
Aspergillus nidulans

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School of Biological Sciences
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Dr David Ellis
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