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Mould Identification: A Virtual Self Assessment

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Unknown 55 = Trichoderma harzianum

Case History
A single blood culture bottle from a 12 year old ALL patient with neutropenia grew the fungus shown below.
Culture

Colonies are fast growing, at first white and downy, later developing yellowish-green to deep green compact tufts, often only in small areas or in concentric ring-like zones on the agar surface.
Microscopy

Conidiophores are repeatedly branched, irregularly verticillate, bearing clusters of divergent, often irregularly bent, flask-shaped phialides. Conidia are mostly green, sometimes hyaline, with smooth or rough walls and are formed in slimy conidial heads (gloiospora) clustered at the tips of the phialides.
Comment: Trichoderma is a very common genus especially in soil and decaying wood. Gliocladium (with strongly convergent phialides) and Verticillium (with straight and moderately divergent phialides) are closely related genera. Trichoderma viride has been reported as a causative agent of pulmonary fungus ball and of CAPD associated peritonitis.

About Trichoderma Back to Virtual Assessment

What is your identification?

Trichoderma harzianum
Paecilomyces variotii
Gliocladium virens

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Dr David Ellis
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