Mould Identification: A Virtual Self Assessment
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Please find additional information below
Unknown = Rhizopus arrhizus
|A 10 year-old girl with idiopathic aplastic anemia presented with pancytopenia and febrile neutropenia. Immunosuppressive therapy included antihymocyte globin, cyclosporin and high dose methylprednisolone. A progressive swelling with blisters and ulceration developed on her right thigh. Direct examination of biopsy tissue revealed abundant filamentous hyphae and all cultures grew the fungus shown below.|
Tissue morphology in zygomycosis showing distinctive infrequently septate thin walled hyphae with focal bulbous dilations and irregular branching, typical for those species belonging to the Mucorales.
|Culture (24 hours growth)|
On Sabouraud's dextrose agar, colonies are very fast growing at 25C, reaching 5-8 mm in height, with some tendency to collapse, white cottony at first, becoming brownish grey to blackish-grey depending on the amount of sporulation.
Sporangiophores up to 1500 um in length and 18 um in width, smooth walled, non-septate, simple or branched, arising from stolons opposite rhizoids usually in groups of 3 or more.
Sporangia are globose, often with a flattened base, greyish black, powdery in appearance, up to 175 um in diameter and many spored. Columella and apophysis together are globose, subglobose or oval, up to 130 um in height and soon collapse to an umbrella-like form after spore release. Sporangiospores are angular, subglobose to ellipsoidal, with ridges on the surface, and up to 8 um in length.
|Growth Temperature Studies|
No growth at 45C; good growth at 37C.
What is your identification?