Mould Identification: A Virtual Self Assessment
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Unknown 20 = Cladophialophora carrionii
|A 46-year-old male, prospector presented with a 6 month history of a white verrucoid cutaneous lesion on his forearm. A biopsy showed the presence of dark brown sclerotic cells and the fungus shown below was isolated.|
H&E stained section showing characteristic dark brown sclerotic cells which divide by binary fission and not by budding. All agents of chromoblastomycosis form these sclerotic bodies in tissue. Direct microscopy of tissue is necessary to differentiate between chromoblastomycosis and phaeohyphomycosis where the tissue morphology of the causative organism is mycelial.
On Sabouraud's dextrose agar, colonies are slow growing, reaching 3-4 cm in diameter after one month, with a compact suede-like to downy surface. Colonies are olivaceous-black in color and have well defined margins.
Microscopic morphology shows a Cladosporium type conidial ontogeny with elongate conidiophores producing branched acropetal chains of smooth-walled conidia, 1.5-3.0 x 2.0-7.0 um in size. Maximum growth temperature 35-37C.
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