Mould Identification: A Virtual Self Assessment
Excellent you have really done well.
Please find additional information below
Unknown 18 = Exophiala dermatitidis
|A 60 year-old male presented with a subcutaneous lesion on the sole of his right foot. A single draining sinus was present which yielded several black grains.Cultures grew the fungus shown below.|
|Culture (note good growth at 40C)|
Colonies are slow growing, initially black and yeast-like, becoming suede-like, olivaceous grey and mould-like with age. Cultures grow at 42C.
An initial yeast-like phase is characterized by unicellular, ovoid to elliptical, budding yeast-like cells. With the development of mycelium, flask-shaped to cylindrical annellides are produced. Spherical phialiees with large, fragile collaretts may also be present (not seen in this strain). Conidia are hyaline to pale brown, one-celled, round to obovoid, 2.0-4.0 x 2.5-6.0 um, smooth-walled and accumulate in slimy balls (glioconidia) at the apices of the phialides or down their sides.
The identity of this isolate was confirmed by sequencing the the D1/D2 region of large subunit rRNA. The strain showed >99.8% identity to numerous E. dermatitidis strains including strains from CBS while it only shows 97.5% ID to Exophiala jeanselmei.
What is your identification?