Mould Identification: A Virtual Self Assessment
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Unknown 27 = Trichophyton tonsurans
|A 40-year-old man presented with a discoloured and thickened finger nail. Nail scrapings were taken and hyphal elements were observed on direct microscopy and the culture shown below was grown.|
T. tonsurans infection of a nail (tinea unguium).
|Direct Microscopy (H&E)|
Hyphal elements of T. tonsurans ramifying through skin scales mounted in 10% KOH and Parker ink solution.
Cultures of T. tonsurans show considerable variation in texture and colour. They may be suede-like to powdery, flat with a raised centre or folded, often with radial grooves. The colour may vary from pale-buff to yellow, which may resemble Epidermophyton floccosum, to dark-brown. The reverse colour varies from yellow-brown to reddish-brown to deep mahogany.
The hyphae are relatively broad, irregular and much branched with numerous septa. Numerous characteristic microconidia varying in size and shape from long-clavate to broad-pyriform are borne at right angles to the hyphae, which often remain unstained by lactophenol cotton blue.
Very occasional smooth, thin-walled, irregular, clavate macroconidia may be present in some cultures (slide 296). Numerous swollen giant forms of microconidia and chlamydoconidia are produced in older cultures.
What is your identification?Trichophyton tonsurans