Mould Identification: A Virtual Self Assessment
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Unknown 29 = Aspergillus nidulans
| Case History | |
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| A 6 year old boy with chronic granulomatous disease presented with chronic pulmonary infiltrates. A needle biopsy showed the presence ofhyphal elements in a Grocott’s methenamine silver (GMS) stained tissue section and the culture shown below was grown. | |
| Direct Microscopy (GMS) | |
Grocott’s methenamine silver (GMS) stained tissue section of lung showing dichotomously branched, septate hyphae typical of Aspergillus.
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| Culture | |
Aspergillus nidulans on Czapek dox agar showing typical plain green colony with dark red-brown cleistothecia developing within and upon the conidial layer. Reverse may be olive to drab-grey or purple-brown.
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| Microscopy | |
Conidial heads of Aspergillus nidulans are short columnar and biseriate. Conidiophores are usually short, brownish and smooth-walled. Conidia are globose and rough-walled.
Cleistothecium of Emericella nidulans (anamorph A. nidulans) showing numerous reddish-brown ascospores .
Cleistothecia of A. nidulans are often surrounded by a mass of Hülle cells which are up to 25 um in diameter.
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Comment: Aspergillus nidulans is a typical soil fungus with a world-wide distribution. It has also been reported as a causative agent of aspergillosis in humans and animals.
What is your identification?
Neosartorya fischeri
Aspergillus flavipes
Aspergillus nidulans
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