Cyberlindnera fabianii
Synonymy:
Candida fabianii.
Cyberlindnera fabianii is a rare cause of candidaemia.
RG-1 organism.
Culture:
Colonies (SDA) white to cream-coloured smooth, glabrous, yeast-like.
Microscopy:
Spheroidal to ellipsoidal budding blastoconidia, 3.0-6.5 x 2-5.5 µm in size. No pseudohyphae are produced. Asci when present, are spherical, containing one to four spherical, finely roughened ascospores.
India ink preparation:
Negative - no capsules present.
Dalmau plate culture:
Spherical to ovoid budding yeast cells and occasional pseudohyphae produced.
Molecular identification:
ITS sequencing recommended.
MALDI-TOF MS:
Able to accurately identify this species.
| Physiological Tests: + Positive, - Negative, v Variable, w Weak, s Slow, nd No Data | |||||||
|---|---|---|---|---|---|---|---|
| Germ Tube | - | L-Sorbose | - | L-Arabinose | - | D-Glucitol | + |
| Fermentation | Sucrose | + | D-Arabinose | - | 𝝰-M-D-Glucoside | + | |
| Glucose | + | Maltose | + | D-Ribose | - | D-Gluconate | + |
| Galactose | - | Cellobiose | + | L-Rhamnose | - | DL-Lactate | - |
| Sucrose | + | Trehalose | + | D-Glucosamime | - | myo-Inositol | - |
| Maltose | +,s | Lactose | - | N-A-D-glucosamine | - | 2-K-D-Gluconate | - |
| Lactose | - | Melibiose | - | Glycerol | + | D-Glucuronate | nd |
| Trehalose | - | Raffinose | + | Erythritol | - | Nitrate | + |
| Assimilation | Melezitose | + | Ribitol | - | Urease | - | |
| Glucose | + | Soluble Starch | + | Galactitol | - | 0.1% Cycloheximide | nd |
| Galactose | - | D-Xylose | + | D-Mannitol | + | Growth at 37C | + |
Key features:
Germ tube negative yeast and sugar assimilation pattern. Molecular identification may be required.
| Antifungal | No | ≤0.03 | 0.06 | 0.125 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | 32 | ≥64 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMB | 1 | 1 | |||||||||||
| FLU | 1 | 1 | |||||||||||
| VORI | 5 | 4 | 1 | ||||||||||
| POSA | 5 | 3 | 2 | ||||||||||
| ITRA | 1 | 1 | |||||||||||
| ANID | 1 | 1 | |||||||||||
| MICA | 4 | 2 | 1 | 1 | |||||||||
| CAS | 1 | 1 | |||||||||||
| 5FC | 1 | 1 |
References
- Arastehfar, A., Fang, W., Al-Hatmi, A.M.S., et al. (2019) Unequivocal identification of an underestimated opportunistic yeast species, Cyberlindnera fabianii, and its close relatives using a dual-function PCR and literature review of published cases. Medical Mycology, 57, 833-840.
- Kurtzman, C.P. (2011) Chapter 42, Lindnera Kurtzman, Robnett & Basehoar-Powers (2008), in Kurtzman, C.P., Fell, J.W. and Boekhout, T. (eds), The Yeasts, a Taxonomic Study, 5th edition, Elsevier B.V., pp. 521-543.
- Park, J.H., Oh, J., Sang, H., et al. (2019) Identification and antifungal susceptibility profiles of Cyberlindnera fabianii in Korea. Mycobiology, 47, 449-456.
- Pfaller, M.A., Rhomberg, P.R., Messer, S.A., et al. (2015) Isavuconazole, micafungin, and 8 comparator antifungal agents' susceptibility profiles for common and uncommon opportunistic fungi collected in 2013: temporal analysis of antifungal drug resistance using CLSI species-specific clinical breakpoints and proposed epidemiological cutoff values. Diagnostic Microbiology and Infectious Diseases, 82, 303-313.